Methods and Techniques in Applied Biology MCQs

Question 1

The technique which involves addition or deletions of genes is

Accepted Answer

Genetic engineering

Answer

Gene therapy

Answer

Gene splicing

Answer

Artificial synthesis

Question 2

In Southern blotting, DNA is fixed on

Accepted Answer

A and B both

Answer

Nitrocellulose

Answer

Nylon membrane

Answer

None of these

Question 3

Which of the following statement is incorrect?

Accepted Answer

None of these

Answer

Inactivation of a gene for determination of gene function of that gene is called gene knockout

Answer

Technique used to construct a clone by overlapping fragments of cloned DNA is called chromosome walking

Answer

A clinical procedure in which a gene or other DNA sequence is used to treat a disease is called gene therapy

Question 4

RF =

Accepted Answer

$$\frac{{{	ext{Distance from origin run by the compound}}}}{{{	ext{Distance from origin run by the solvent}}}}$$

Answer

$$\frac{{{	ext{Distance from last point to origin}}}}{{{	ext{Distance from origin run by the solvent}}}}$$

Answer

$$\frac{{{	ext{Distance from origin run by the solvent}}}}{{{	ext{Distance from origin run by the compound}}}}$$

Answer

None of the above

Question 5

The sensitivity of ELISA is

Accepted Answer

10-9 g/ml

Answer

10-6 g/ml

Answer

10-7 g/ml

Answer

10-12 g/ml

Question 6

Hybridoma technology is used in

Accepted Answer

Synthesis of monoclonal antibodies

Answer

Synthesis of haemoglobin

Answer

Production of alcohol

Answer

Production of somatic hybrids

Question 7

Which statement about restriction enzymes are false?

Accepted Answer

Restriction enzymes are exonucleases rather than endonucleases

Answer

Restriction enzymes cut DNA at specific sequence called recognition sites

Answer

A restriction enzyme always cut DNA to leave the same sequence at the ends

Answer

Some restriction enzymes cut the two DNA strands at slightly different points within their recognition site to make a 'sticky' end

Question 8

Free amino acids can be determined with the help of

Accepted Answer

Auxotrophic bacteria

Answer

Heterotrophic bacteria

Answer

Algae

Answer

Fungi

Question 9

Reverse genetics means

Accepted Answer

Finding the gene responsible for a trait

Answer

Finding the functions of a ORF

Answer

RNA dependent DNA synthesis

Answer

Converting somatic cell to a germ cell