Methods and Techniques in Applied Biology MCQs

Question 1

Northern blotting is developed by

Accepted Answer

Alwine et al

Answer

Ed Southern

Answer

Thomas et al

Answer

E. Northern

Question 2

Which of the following is incorrect?

Accepted Answer

None of the above

Answer

The broken cell preparation is loosely referred to as cell-free preparation or HOMOGENATE

Answer

SUPERNATANT is that part of the cell material not sedimented by a centrifugal force known to sediment any subcellular structure

Answer

A BLENDER consist of a glass vessel fitted with rotating knives

Question 3

A gene that does not contain introns has an Eco RI site just down stream of the translational termination codon in the 3' untranslated region. The size of the gene is 4 Kbp. The length of 3' UTR is 2 Kbp, the 5' UTR is 1 Kbp and that the open reading frame is 1kbp. Upon probing a southern blot of an Eco RI digested genomic DNA with the radioactively labelled fragment containing only the open reading frame, the size of the band detected by hybridization would be

Accepted Answer

1 Kbp

Answer

> 2 Kbp

Answer

0.5 Kbp

Answer

1.5 Kbp

Question 4

Cells deficient in hypoxanthine guanine phospho-ribosyl transferase (HGPRT)

Accepted Answer

Synthesis of purine deoxynucleotides by de novo path way

Answer

Synthesis of purine deoxynucleotides by salvage pathway

Answer

Supply of hypoxanthine in the culture medium

Answer

Supply of thymidine in the culture medium enzyme rely on

Question 5

Which of the following statements gives a correct explanation for the use of vectors containing drug resistance genes in the cloning of recombinant DNA (cDNA) molecules?

Accepted Answer

Entry of the vector containing the cDNA and the drug resistance genes into the host cell renders the latter identifiable as it is now resistant to antibiotic drugs

Answer

The products of the drug resistance genes protect the cDNA from destruction by the host cells

Answer

The drug resistance genes provide additional base sequences that enable the vector to accommodate larger inserts of cDNA

Answer

The cloned cDNA imparts drug resistance upon any cellular system with which it is used

Question 6

There are two different ways of determining the nucleotide sequence of a nucleic acid: the chemical quencing (Maxam-Gilbert) method and the enzymatic sequencing (Sanger) method. The basic principle/advantage of the Sanger method is

Accepted Answer

The ability to 'sequence' both strands of the DNA duplex simultaneously

Answer

The differential interaction of the bases with particular dyes

Answer

Extension of a synthetic primer and reliable termination of DNA repair synthesis

Answer

The correlation of restriction sites with the end-label of the DNA

Question 7

The ability to clone an adult animal from the nucleus of a differentiated somatic cell proves that

Accepted Answer

The nuclei of somatic cells contain all the genetic information present in a fertilized egg

Answer

The germ line and soma are identical

Answer

Genetic engineering is a powerful technique

Answer

Animals are not all that different from plants

Question 8

For protoplast fusion to be successful in plant cells.

Accepted Answer

DNA between the two cells should be compatible

Answer

Fusion agents other than polyethylene glycol should be used

Answer

Cell wall of the two strains of cells should not be damaged

Answer

Osmolarity of the medium is not important

Question 9

The process used for separation of protein in polyacrylamide gel is called:

Accepted Answer

Western blotting

Answer

Southern blotting

Answer

Northern blotting

Answer

Eastern blotting