Methods and Techniques in Applied Biology MCQs

Question 1

Which of the following would not possible to address using a Northern Blot?

Accepted Answer

Location of restriction sites in a particular gene

Answer

Spatial expression of a particular gene

Answer

Temporal expression of a particular gene

Answer

mRNA size

Question 2

In southern blotting . . . . . . . . is separated by gel electrophoresis

Accepted Answer

DNA

Answer

m-RNA

Answer

t-RNA

Answer

Protein

Question 3

A restriction enzyme AluI with a 4-bp recognition site produces restriction fragments that should be

Accepted Answer

256 bp

Answer

4096 bp

Answer

16 bp

Answer

436 bp

Question 4

Terminal transferase is used

Accepted Answer

To add base at the 3' end of the DNA

Answer

To add base at the 5' end of the DNA

Answer

To carry out nick translation

Answer

To transfer phosphate at the 3' end of the DNA

Question 5

Mung bean nuclease could be used for

Accepted Answer

Trimming single stranded regions in DNA

Answer

DNA synthesis

Answer

Nucleotide hydrolysis

Answer

Removal of phosphate groups from the ends of the DNA

Question 6

Many plasmids have Ampicillin marker. This implies

Accepted Answer

The plasmid contains the gene encoding β-lactamase

Answer

The plasmids contain genes for ampicillin biosynthesis

Answer

Ampicillin is required for bacterial growth after transformation

Answer

Ampicillin is essential for cell survival

Question 7

DNA synthesis can be measured by estimating incorporation of radio-labelled

Accepted Answer

Thymidine

Answer

Uracil

Answer

Ribose sugar

Answer

Adenine

Question 8

In agarose gel electrophoresis

Accepted Answer

Ethidium bromide can be used to visualize the DNA

Answer

DNA migrates towards the negative electrode

Answer

Supercoiled plasmids migrate slower than their nicked counterparts

Answer

Larger molecules migrate faster than smaller molecules

Question 9

A cloning vector consisting of CoS site inserted in a plasmid, used to clone DNA fragments of λ phage is

Accepted Answer

Cosmid

Answer

Phage mid

Answer

Plasmid

Answer

YAC