Methods and Techniques in Applied Biology MCQs

Question 1

Genetically engineered male sterile crop plants may be produced by inserting

Accepted Answer

BT toxin gene

Answer

Barnase gene

Answer

Lectin gene

Answer

Chitinase gene

Question 2

Two restriction enzymes A and B have eight and four base pairs as their recognition sites respectively. The ratio of the number of fragments that they will generate on restriction digestion of a genomic DNA of E.coli is approximately

Accepted Answer

1 : 26

Answer

4 : 8

Answer

8 : 4

Answer

1 : 64

Question 3

Which of the following is a desirable characteristic for a cloning plasmid?

Accepted Answer

All of the above

Answer

A site at which replication can be initiated

Answer

One or more unique restriction endonuclease sites

Answer

One or more antibiotic-resistance or drug resistance genes

Question 4

Which of the following could not possibly give rise to a restriction fragment length polymorphism (RFLP)?

Accepted Answer

An error in RNA splicing that mistakenly removes an exon during RNA processing

Answer

A missense mutation within the protein coding region of a gene

Answer

A silent mutation within the protein coding region of a gene

Answer

A single base change within the intron sequence of a gene

Question 5

Which of the following is a semi-continuous fermentation in which only a small amount of substrate is available at the beginning of the process?

Accepted Answer

Fed-batch fermentation

Answer

Feed-back fermentation

Answer

Reduced Fermentation

Answer

None of the above

Question 6

Which statement correctly describes sequential steps in cDNA cloning?

Accepted Answer

Reverse transcription of mRNA, second strand synthesis, cDNA end modification, ligation to vector

Answer

mRNA preparation, cDNA synthesis using reverse transcription, second strand synthesis using terminal transferase, ligation to vector

Answer

mRNA synthesis using RNA polymerase, reverse transcription of mRNA, second strand synthesis, ligation to vector

Answer

Double stranded cDNA synthesis, restriction enzyme digestion, addition of linkers, ligation to vector