Methods and Techniques in Applied Biology MCQs

If the linear double stranded genomic DNA of bacteriophage T4 is heated and cooled, one gets both linear and circular molecules because,

The restriction end onuclease Eco521 recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco521?

The genome sequencing of rice is important because

Match List-I (cloning vector) with List-II (character) and select the correct answer: List-I: Cloning vector List-II: Character a. Yeast episomal plasmid (YEp) 1. Integrates with host chromosome for replication b. Yeast integrative plasmid (YIp) 2. Carries a chromosomal origin of replication c. Yeast replicative plasmid (YRp) 3. Carries origin of replication of the 2 mm plasmid

Which of the following methods would give you the most precise and accurate information about where and when a given gene is expressed?

Which of the following are not valid methods of labelling duplex DNA?

BAC, which can be used to clone large DNA fragments, is derived from

Restriction endonucleases hydrolyzes polynucleotide from

ELISA is used to detect viruses where the key reagent is