Molecular Tools in Biotechnology MCQs

Which of the following fluorescent probes is used to monitor the progress of amplification in Real time PCR?

Match items in Group I with correct options from those given in Group II. Group I Group II P. VNTR sequence 1. Gene regulation on the same chromosome Q. Leader sequence 2. Ribosome binding site R. SD sequence 3. DNA fingerprinting S. cis-acting sequence 4. Functions in attenuation

For the sequence of dsDNA given below, identify the set of primers required to amplify this DNA by PCR. 3' GACTCCA . . . . . . . . TACAACC 5' 5' CTGAGGT . . . . . . . . ATGTTGG 3'

Choose the appropriate pair of primers to amplify the following DNA fragment by the polymerase chain reaction (PCR). 5'-GACCTGTGG . . . . . . . . ATACGGGAT-3' 3'-CTGGACACC . . . . . . . . TATGCCCTA-5' Primers P. 5'-GACCTGTGG-3' Q. 5'-CCACAGGTC-3' R. 5'-TAGGGCATA-3' S. 5'-ATCCCGTAT-3'

Protein binding regions of DNA are identified by one of the following techniques.

Nick translation of DNA is a method for making DNA probes. Identify from below what is not required for nick translation method.

Match the reagents in Group I with their preferred cleavage sites in Group II. Group I Group II P. Cyanogen bromide 1. Carboxyl side of methionine Q. o-Iodosobenzoate 2. Amino side of methionine R. Hydroxylamine 3. Carboxyl side of tryptophan S. 2-Nitro-5-thiocyanobenzoate 4. Amino side of cysteine 5. Asparagine-glycine bonds

The mobility of DNA in agarose gel electrophoresis is solely based on its

A polymerase chain reaction was performed beginning with 400 template DNA molecules in a 100 μL reaction. After 20 cycles of polymerase chain reaction, how many molecules of the amplified product will be present in 0.1 μL of reaction?