Molecular Tools in Biotechnology MCQs

Question 1

Restriction endonucleases from two different organisms that recognize the same DNA sequence for cleavage are called

Accepted Answer

Isoschizomers

Answer

Isozymes

Answer

Concatemers

Answer

Palindromes

Question 2

Protein-DNA interactions in vivo can be studied by

Accepted Answer

Chromatin immunoprecipitation assay

Answer

Gel shift assay

Answer

Southern hybridization

Answer

Fluorescence in situ hybridization assay

Question 3

The DNA sequence shown below is to be amplified by PCR: 5' GCTAAGATCTGAATTTTCC . . . . . . . . TTGGGCAATAATGTAGCGC 3' 3' CGATTCTAGACTTAAAAGG . . . . . . . . AACCCGTTATTACATCGCG 5' Which one of the following pair of primers can be used for this amplification?

Accepted Answer

5' GCTAAGATCTGAATTTTC 3' and 5' GCGCTACATTATTGCCCA 3'

Answer

5' GGAAATTCAGATCTTAGT 3' and 5' TGGGCAATAATGTAGCGC 3'

Answer

5' CGGAAATTCAGATCTTAG 3' and 5' GCGCTACATTATTGCCCA 3'

Answer

5' GCTAAGATCTGAATTTTC 3' and 5' TGGGCAATAATGTAGCGC 3'

Question 4

For site-directed mutagenesis, which one of the following restriction enzymes is used to digest methylated DNA?

Accepted Answer

DpnI

Answer

KpnI

Answer

XhoI

Answer

MluI