Molecular Tools in Biotechnology MCQs

A proto-oncogene is suspected to have undergone duplication in a certain type of cancer. Of the following techniques, which one would verify the gene duplication?

All of the following are true about DNA microarray technology except

Expressed Sequence Tag is defined as

Which one of the following techniques is used to monitor RNA transcripts, both temporally and spatially?

CRISPR-Cas system is associated with

Identify the correct statements. P. 5' and 3' ends of the transcripts can be mapped by utilizing polymerase chain reaction. Q. S1 nuclease can cleave the DNA strand of a DNARNA hybrid. R. T4 polynucleotide kinase is used for labeling 3' end of DNA. S. Baculovirus (Autographa californica) can be used as an insect expression vector.

W, X and Y are the intermediates in a biochemical pathway as shown below: $$S \cdot \cdot \cdot \cdot \cdot \cdot \cdot \cdot \to W \to X \to Y \to Z$$ Mutants auxotrophic for Z are found in four different complementation groups, namely Z1, Z2, Z3 and Z4. The growth of these mutants on media supplemented with W, X, Y or Z is shown below (Yes: growth observed: No: growth not observed): Mutants Media Supplemented with Mutants W X Y Z Z1 No No Yes Yes Z2 No Yes Yes Yes Z3 No No No Yes Z4 Yes Yes Yes Yes What is the order of the four complementation groups in terms of the step they block?

For amplification of GC rich sequences by polymerase chain reaction, identify the reagent that hinds and stabilizes AT sequences and destabilizes GC regions.

In order to identify the person who committed a crime, forensic experts will need to extract DNA from the tissue sample collected at the crime scene, and conduct one of the following procedures for DNA fingerprinting analysis