Molecular Tools in Biotechnology MCQs

A polymerase chain reaction (PCR) was set up with the following reagents: DNA template, Taq polymerase, buffer, dNTPs, and Mg2+. Which one of the following is missing in the reaction mixture?

Enzyme used in 'cycle' sequencing of DNA is

Which one of the following is not a requirement for a PCR reaction?

Which of the following statements about gene therapy are correct? P. Affected individuals, but not their progeny, can be cured through germline gene therapy. Q. Affected individuals, as well as their progeny, can be cured through germline gene therapy. R. Affected individuals, but not their progeny, can be cured through somatic gene therapy. S. Affected individuals, as well as their progeny, can be cured through somatic gene therapy.

Which of the following factors affect the fidelity of DNA polymerase in polymerase chain reaction? P. Mg2+ concentration Q. pH R. Annealing temperature

The heterozygosity of any locus can be ascertained by

For PCR to amplify the sequence ATCTTCTACG . . . . . . . . AAGCPTGCGG TAGAAGATGC . . . . . . . .TTCGAACGCC The required primers are

In DNA sequencing reactions using the chain termination method, the ratio of ddNTPs to dNTPs should be

In human populations 4% of the individuals are homozygous recessive to a specific RFLP marker. What percentage of individuals does you expect to be heterozygous for this trait?