Enzyme Engineering in Biotechnology MCQs

Question 1

Which type of restriction enzymes are commonly used in rDNA technology

Accepted Answer

Type II

Answer

Type I

Answer

Type III

Answer

Type IV

Question 2

For every, 1000 fold increase in pressure, the Km is . . . . . . . .

Accepted Answer

Reduced to half

Answer

Doubled

Answer

Reduced to zero

Answer

Increased by 10 folds

Question 3

In this below equation, Enzyme Y belongs to which class of enzymes? $$A - X + {H_2}O\xrightarrow[{Enzyme\,Y}]{}X - OH + AH$$

Accepted Answer

Hydrolases

Answer

Peroxidase

Answer

Pectinase

Answer

Aldolase

Question 4

Which of the following is not a physical method for extraction of enzymes?

Accepted Answer

Osmotic shock

Answer

Ultrasonication

Answer

Homogenization

Answer

Freezing technique

Question 5

The following rate equation is given by . . . . . . . . inhibition. $$V = {{\left( {{{{V_{\max }}} \over {1 + {{\left[ I \right]} \over {K{'_i}}}}}} \right)\left[ S \right]} \over {\left( {{{{K_m}} \over {1 + {{\left[ I \right]} \over {K{'_i}}}}}} \right) + S}}$$

Accepted Answer

Uncompetitive

Answer

Mixed

Answer

Competitive

Answer

Non-competitive

Question 6

Which enzyme hydrolysis casein?

Accepted Answer

Chymosin

Answer

Glucose oxidase

Answer

Alanine aminotransferase

Answer

Histidase

Question 7

Which chemical is used for extracting enzymes by osmotic shock treatment?

Accepted Answer

1.4% buffered sucrose

Answer

EDTA

Answer

NaOH

Answer

SDS

Question 8

Which of the oxidoreductases are involved in oxygen transfer form molecular oxygen?

Accepted Answer

Oxygenases

Answer

Peroxidases

Answer

Oxidases

Answer

Dehydrogenases

Question 9

What is the rate of denaturation kd, if the half-life of an enzyme catalyzed reaction is 15.6 mins?

Accepted Answer

4.4*10-2/min

Answer

4.4*10-2/sec

Answer

0.4*102/min

Answer

0.4*102/sec