Enzyme Engineering in Biotechnology MCQs

Question 1

Which class of enzymes contain relatively small group of enzymes?

Accepted Answer

D-xylose ketol-isomerase

Answer

Ligases

Answer

Oxidoreductase

Answer

Glutathione synthetase

Question 2

Inhibition of lactase by galactose is an example of which kind of inhibition?

Accepted Answer

Competitive inhibition

Answer

Uncompetitive inhibition

Answer

Mixed inhibition

Answer

Substrate inhibition

Question 3

Enzymes which are secreted outside the body cells are referred to as . . . . . . . . enzymes.

Accepted Answer

Extracellular

Answer

Intracellular

Answer

Cytoplasmic

Answer

Mitochondrial

Question 4

Histidase: Lyases :: Chymosin: . . . . . . . .

Accepted Answer

Hydrolases

Answer

Ligases

Answer

Isomerases

Answer

Transferases

Question 5

An uncatalyzed reaction involved:

Accepted Answer

High activation energy

Answer

Low activation energy

Answer

Balanced activation energy

Answer

All of these

Question 6

Which of the following methods cannot be used to isolate enzymes from cell free extract?

Accepted Answer

Homogenization

Answer

Use of factors like pH and ionic strength

Answer

Use of chemicals

Answer

Use of temperature factor

Question 7

Pick odd one out.

Accepted Answer

Triton-X-100

Answer

Cetyl triethyl ammonium bromide

Answer

Sodium lauryl sulphate (SLS)

Answer

Cetyl trimethyl ammonium bromide

Question 8

Pectinases belong to which class of enzyme?

Accepted Answer

Lyases

Answer

Ligases

Answer

Hydrolases

Answer

Transferases

Question 9

In competitive inhibition, what happens to Vmax and Km if [I] = Ki?

Accepted Answer

Vmax is unchanged and Km increases 2Km

Answer

Lowers to 0.5 Vmax and 0.5 Km

Answer

Lowers to 0.5 Vmax and Km remains unchanged

Answer

Lowers to 0.67 Vmax and Km increases to 2Km