Enzyme Engineering in Biotechnology MCQs

Question 1

Which of the following is the best method for isolating enzymes from cell free extract?

Accepted Answer

Chemical treatment

Answer

pH treatment

Answer

Temperature treatment

Answer

Osmotic shock

Question 2

Absolute specificity, group specificity and optical specificity are observed by chemical catalyst.

Accepted Answer

False

Answer

True

Question 3

Which of the following enzyme is used to join two DNA molecule

Accepted Answer

Ligases

Answer

Nuclease

Answer

Restriction enzymes

Answer

Lyases

Question 4

The rate of enzyme catalyzed reaction decreases with an increase in ionic strength if the charges are identical.

Accepted Answer

False

Answer

True

Question 5

. . . . . . . . is an example of anionic detergent.

Accepted Answer

Sodium dodecyl sulphate (SDS)

Answer

Tween

Answer

Cetyl triethyl ammonium bromide

Answer

Triton-X-100

Question 6

Which among them is not attribute of enzymes:

Accepted Answer

Consumed in reaction

Answer

Specific in nature

Answer

Protein in chemistry

Answer

Increased rate of reaction

Question 7

The rate equation for . . . . . . . . inhibition is given by $$V = {{{V_{\max }}\left[ S \right]} \over {{K_m}\left( {1 + {{\left[ P \right]} \over {{K_p}}}} \right) + \left[ S \right]}}.$$

Accepted Answer

Product

Answer

Substrate

Answer

Non-competitive

Answer

Competitive

Question 8

The equation $${t_{{1 \over 2}}} = {{0.693} \over {{k_d}}}$$ represents . . . . . . . .

Accepted Answer

Half-life

Answer

Arrhenius equation

Answer

Lineweaver Burk equation

Answer

Gibbs-Helmholtz equation

Question 9

Koshland proposed which model:

Accepted Answer

Induced fit model

Answer

Fluid mosaic model

Answer

Lock and key model

Answer

Reflective index model