Enzyme Engineering in Biotechnology MCQs

Question 1

Extraction of enzymes by physical methods include the usage of alkalis, EDTA, lysozyme, etc.

Accepted Answer

False

Answer

True

Question 2

The phenomenon wherein the osmotic pressure created due to presence of solutes inside and outside the cells will be equal is referred to as . . . . . . . .

Accepted Answer

Isotonic condition

Answer

Hypertonic condition

Answer

Hypotonic condition

Answer

Osmotic shock

Question 3

This statement about enzymes is true:

Accepted Answer

All of these

Answer

Enzymes accelerate reactions by lowering the activation energy

Answer

Enzymes are proteins whose three-dimensional form is key to their function

Answer

Enzymes do not alter the overall change in free energy for a reaction

Question 4

Mercury causes irreversible inhibition.

Accepted Answer

True

Answer

False

Question 5

Homogenization utilizing . . . . . . . . is one where in manual pressure is created for cell lysis.

Accepted Answer

Mortar and pestle

Answer

Ultrasonicator

Answer

Freezer

Answer

French press

Question 6

Enzyme catalysing rearrangement of atomic grouping without altering molecular weight or number of atom is:

Accepted Answer

Isomerase

Answer

Ligase

Answer

Oxidoreductase

Answer

Hydrolase

Question 7

Enzyme which can be extracted from mammary gland is . . . . . . . .

Accepted Answer

Acetyl CoA carboxylase

Answer

Pectinase

Answer

Glucornidase

Answer

Lipase

Question 8

. . . . . . . . is a phenomenon of assuming frequencies more than 20 kHz which is above the human audibility.

Accepted Answer

Ultrasonics

Answer

Homogenization

Answer

Alkali treatment

Answer

Freezing

Question 9

Increase or decrease in . . . . . . . . leads to protonation or deprotonation of bases which leads to melting of DNA in turn isolating the enzyme after centrifugation.

Accepted Answer

pH

Answer

Temperature

Answer

Ionic strength

Answer

Detergent use