Gene Transfer in Plants MCQs

Question 1

Opine synthesis is the property

Accepted Answer

both (a) and (b)

Answer

conferred to plant cells when it transformed by Agrobacterium tumefaciens

Answer

determined by the bacteria Agrobacterium tumefaciens

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of normal plant cells

Question 2

Virulent strains of Agrobacterium contain large Ti-plasmids, which are responsible for the DNA transfer and subsequent disease symptoms. It has been shown that Ti-plasmids contain

Accepted Answer

two sets of sequence necessary for gene transfer

Answer

one set of sequence necessary for gene transfer

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three sets of sequence necessary for gene transfer

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four sets of sequence necessary for gene transfer

Question 3

The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as

Accepted Answer

border sequences

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cos site

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flanking sequences

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transfer sequences

Question 4

T-DNA transfer and processing into plant genome requires products of which of the following genes?

Accepted Answer

All of these

Answer

vir A,B

Answer

vir G,C

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vir D,E

Question 5

Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

Accepted Answer

pBR 322 based plasmid vector

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pRK 2013

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pRN 3

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all of these

Question 6

The transfer of intermediate vectors into Agrobacterium are brought about by

Accepted Answer

triparental mating

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transformation

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biparental mating

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transduction

Question 7

In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by

Accepted Answer

vir D

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vir A

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vir B

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vir C

Question 8

Microinjection involves

Accepted Answer

injection with needle having diameter greater than cell diameter

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injection of large amount of DNA

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injection of DNA into bigger cells

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all of the above

Question 9

Which of the following are used as selection marker for the cells transformed with Agrobacterium?

Accepted Answer

any of the above

Answer

Neomycin phosphotransferase

Answer

Streptomycin phosphotransferase

Answer

Hygromycin phosphotransferase