Gene Transfer in Plants MCQs

Question 1

Liposomes mediated gene transfer in plants involves

Accepted Answer

all of the above

Answer

plasmid DNA enclosed in a lipid bag

Answer

fusion of liposomes with protoplast

Answer

use of polyethylene glycol (PEG)

Question 2

Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?

Accepted Answer

Both (a) and (b)

Answer

Acetosyringone

Answer

α-hydroxy syringone

Answer

None of these

Question 3

Opines that are present in crown gall tumour include

Accepted Answer

all of these

Answer

octopine

Answer

nopaline

Answer

agropine

Question 4

Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?

Accepted Answer

either (a) or (b)

Answer

pRK 2013

Answer

pRN 3

Answer

none of these

Question 5

Which of the following is true about T DNA?

Accepted Answer

Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA

Answer

Integration of T DNA occurs only at one specific sites in the plant nuclear DNA

Answer

Integration of T DNA occurs at two specific sites in the plant nuclear DNA

Answer

Integration of T DNA occurs at one site that may be random in the plant nuclear DNA

Question 6

Which of the following is not true about the direct repeats flanking T-DNA?

Accepted Answer

These repeats are transferred intact to the plant genome

Answer

They are conserved between nopaline and octopine Ti-plasmids

Answer

These are important in integration mechanism

Answer

all of the above

Question 7

The left segment of octopine T-DNA (TL) is necessary for

Accepted Answer

tumour formation

Answer

enzymes for agropine biosynthesis

Answer

conjugative transfer

Answer

all of these

Question 8

Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

Accepted Answer

It employs needle with diameter greater than cell diameter

Answer

It is carried out with the help of micromanipulator

Answer

The recipient cells are immobilized on artificial support or artificially bound to substarate

Answer

all of the above

Question 9

The right segment of octopine T-DNA (TR) is necessary for

Accepted Answer

enzymes for agropine biosynthesis

Answer

tumour formation

Answer

conjugative transfer

Answer

all of these