Gene Transfer in Plants MCQs

Question 1

Virulence trait of Agrobacterium tumefaciens is borne on

Accepted Answer

tumour inducing plasmid DNA

Answer

chromosomal DNA

Answer

both chromosomal and plasmid DNA

Answer

cryptic plasmid DNA

Question 2

The size of the virulent plasmid of Agrobacterium tumefaciens is

Accepted Answer

140-235 kb

Answer

40-80 kb

Answer

80-120 kb

Answer

>235 kb

Question 3

Which of the following is not true about the helper plasmids?

Accepted Answer

These can replicate in Agrobacterium

Answer

These help in the mediating conjugation of intermediate vectors

Answer

These can't replicate in Agrobacterium

Answer

All of the above

Question 4

Which technique is used to introduce genes into dicots?

Accepted Answer

Ti plasmid infection

Answer

electroporation

Answer

particle acceleration

Answer

microinjection

Question 5

Direct DNA uptake by protoplasts can be stimulated by

Accepted Answer

polyethylene glycol (PEG)

Answer

decanal

Answer

luciferin

Answer

all of these

Question 6

In a plant tumour cell

Accepted Answer

only a small specific segment of callus T DNA is incorporated

Answer

complete Ti plasmid is incorporated in plant nuclear DNA

Answer

different parts of the Ti-plasmid are incorporated

Answer

may vary from plant to plant

Question 7

Co-integrating transformation vectors must include a region of homology in

Accepted Answer

between vector plasmid and Ti-plasmid

Answer

the vector plasmid

Answer

the Ti-plasmid

Answer

none of these

Question 8

Crown gall tissue

Accepted Answer

both (a) and (b)

Answer

can be cultivated in vitro in absence of bacteria

Answer

retains tumorous properties when cultivated

Answer

shows tumorous properties only in presence of bacteria

Question 9

Integrated nopaline T-DNA occurs as

Accepted Answer

single segment

Answer

two segments

Answer

three segments

Answer

four segments