Recombinant DNA Technology MCQs

Question 1

In order to insert a foreign gene into a plasmid, both must __________

Accepted Answer

be cut by the same restriction enzyme

Answer

have identical DNA sequences

Answer

originate from the same type of cell

Answer

be of the same length

Question 2

Which of the following genetic diseases would be amenable to genetic engineering?

Accepted Answer

Cystic fibrosis

Answer

Down's syndrome

Answer

Muscular dystrophy

Answer

Cri du Chat

Question 3

X-rays cause

Accepted Answer

ionization of water in the cell

Answer

the formation of thymine dimers

Answer

heat

Answer

none of the above

Question 4

The order for the construction of a cDNA fragment from mRNA is to

Accepted Answer

bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

Answer

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

Answer

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase

Answer

bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

Question 5

The TP53 gene of chromosome 17 codes for a protein __________ .

Accepted Answer

involved in the regulation of the cell cycle

Answer

that plays a role in the digestive process

Answer

involved in glucose transport

Answer

that is like a white blood cell protein

Question 6

Why is golden rice pale yellow in color?

Accepted Answer

It is rich in beta-carotene.

Answer

It is rich in chlorophyll a.

Answer

It is rich in chlorophyll b.

Answer

It is rich in phycobilins.

Question 7

Which type of restriction enzymes do not usually require ATP?

Accepted Answer

Type II

Answer

Type I

Answer

Type III

Answer

Type IV

Question 8

An example of a restriction fragment length polymorphism is

Accepted Answer

different length fragments of DNA resulting from loss or gain of a restriction site

Answer

an Eco RI cuts DNA at a different sequence than Hind III

Answer

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

Answer

all of the above

Question 9

Knockout mice are created by

Accepted Answer

transfecting embryonic stem cells with an altered gene sequence

Answer

mutagenizing a mouse and selecting for mutant offspring

Answer

creating a chimera by fusing cells from two different cell lines

Answer

infecting the mouse with a retrovirus