Recombinant DNA Technology MCQs

Question 1

When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .

Accepted Answer

genetic drift

Answer

assortative mating

Answer

inbreeding

Answer

heterosis

Question 2

Which of the following is correct in terms of determination of location of genetic traits?

Accepted Answer

Known protein coding sequences are too far apart to allow linkage determination for most new genes

Answer

Restriction sites allow DNAs to be digested

Answer

Protein-coding genes are always associated with a restriction pattern

Answer

None of the above

Question 3

In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.

Accepted Answer

negative ... positive

Answer

basic ... acidic

Answer

long ... short

Answer

positive to negative

Question 4

Restriction enzymes

Accepted Answer

all of the above

Answer

protect bacteria from viral infection

Answer

cut DNA in a staggered fashion

Answer

cut DNAs producing a blunt end

Question 5

First discovered, Type II restriction endonuclease was

Accepted Answer

Hind II

Answer

Hinf I

Answer

Eco K

Answer

EcoRI

Question 6

Which of the following techniques can be used to determine the defective gene and for developing cancer?

Accepted Answer

Southern blot

Answer

Western blot

Answer

Northern blot

Answer

Eastern blot

Question 7

The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

Accepted Answer

seems unlikely

Answer

would be of no concern if it occurred.

Answer

has occurred

Answer

can never occur

Question 8

Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

Accepted Answer

all of the above

Answer

in both cases molecules migrate to the anode

Answer

both techniques rely on a constant charge to mass ratio

Answer

both techniques utilize the sieving properties of gels

Question 9

Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

Accepted Answer

short sequences that will hybridize only to a specific base sequence

Answer

copies of the gene with an altered sequence so that a restriction site is inserted

Answer

mutagenized copies of a gene

Answer

PCR-amplified variable numbers of tandem repeats (VNTRs)