Recombinant DNA Technology MCQs

Question 1

A plasmid

Accepted Answer

all of the above

Answer

is a circular DNA molecule

Answer

always contains an origin of replication

Answer

usually contains one or more restriction sites

Question 2

Under which of the following conditions would population gene frequencies remain the same?

Accepted Answer

Random mating

Answer

Selection for homozygotes

Answer

Small population size

Answer

Active migration between groups

Question 3

Plant transformation can be obtained by

Accepted Answer

shooting DNA into plant cells with a gun

Answer

combining plant and animal cells in culture

Answer

using the E. coli bacterium to infect plant roots

Answer

infecting plants with a tobacco mosaic virus

Question 4

An expression vector

Accepted Answer

all of the above

Answer

always contains an origin of replication

Answer

usually contains a gene that confers antibiotic resistance to the bacterial host

Answer

always contains DNA segments for the regulation of mRNA production

Question 5

The unpaired nucleotides produced by the action of restriction enzymes are referred to have

Accepted Answer

sticky ends

Answer

single strands

Answer

restriction fragments

Answer

ligases

Question 6

Which of the following is not true of small inbreeding populations?

Accepted Answer

Mutation is increased

Answer

They tend to loose genetic diversity

Answer

There is an increased incidence of recessive diseases

Answer

Alleles may become fixed

Question 7

Which of these genes codes for a protein that plays a role in growth?

Accepted Answer

GH1

Answer

DCP1

Answer

SCLC6A4

Answer

KRTHA1

Question 8

Which of the following pair will produce complementary sticky ends?

Accepted Answer

Sau 3A & Bam HI

Answer

Eco RI & MspI

Answer

Msp I & HPA II

Answer

Mbo I & Sau 3A

Question 9

A human cDNA library

Accepted Answer

contains DNA for virtually all of the human proteins in vectors

Answer

contains DNA for specific human proteins

Answer

cannot be used to obtain human genes because it would be radioactive

Answer

none of the above