Recombinant DNA Technology MCQs

Question 1

You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because

Accepted Answer

Restriction endonucleases do not cut RNA, and this virus has an RNA genome

Answer

There are no introns in the genome

Answer

The introns contain the recognition sites and have already been spliced out

Answer

All of restriction fragments are too small to detect

Question 2

Which one of the following features is not required in a prokaryotic expression vector?

Accepted Answer

CMV promoter

Answer

oriC

Answer

Selection marker

Answer

Ribosome binding site

Question 3

Yeast artificial chromosomes (YAC's) are used for cloning

Accepted Answer

Large segments of DNA

Answer

mRNA

Answer

Bacterial DNA

Answer

Yeast DNA

Question 4

In baculovirus expression vector, foreign genes are expressed from the promoter of

Accepted Answer

Polyhedrin gene

Answer

Bacteriophage T7 gene

Answer

E. coli lacZ gene

Answer

Yeast phosphoglycerate kinase gene

Question 5

Which of these restriction enzymes produce blunt ends?

Accepted Answer

EcoRV

Answer

SaII

Answer

XhoI

Answer

HindIII

Question 6

The RP13 gene of chromosome 17 codes for a protein __________ .

Accepted Answer

involved in eye development

Answer

involved in glucose transport

Answer

that is a component of hair and nails

Answer

involved in the determination of personality

Question 7

Isoschizomers recognize

Accepted Answer

same recognition site and recognition sequence

Answer

same recognition sequence but different recognition site

Answer

same recognition site and different recognition sequence

Answer

different recognition site and different recognition sequence

Question 8

Restriction enzymes are named for

Accepted Answer

the bacterium they are derived from

Answer

the person who discovered

Answer

the viral DNA that they attack

Answer

none of the above

Question 9

Which of these genes codes for a protein that plays a role in white blood cell function?

Accepted Answer

MPO

Answer

DCP1

Answer

GLUT4

Answer

RP13