Recombinant DNA Technology MCQs

Match the items in Group I with their functions in Group II. Group I Group II P. rol genes 1. Food and energy source Q. Opines 2. Tumor formation R. Virulence genes 3. Hairy root induction S. aux and cyt genes 4. T-DNA transfer and integration

A vector derived from which one of the following viruses is used for high-frequency genomic integration of a transgene in animal cells?

In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to P. Crown gall disease in plants. Q. Hairy root disease in plants. R. Transfer of T-DNA into the plant chromosome. S. Transfer of Ri-plasmid into the plant cell.

Mung bean nuclease could be used for

Human genome sequencing project involved the construction of genomic library in

Which of the following processes require energy?

A plasmid cloning vector should contain all of the following sequences except

"A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRI individually. Restriction digestion yielded following fragments. Plasmid + HindIII → 1200bp and 3800bp Plasmid + BamHI → 5000bp Plasmid + EcoRI → 2500bp The number of sites for EcoRI, BamHI and HindIII present on this plasmid are"

Consider the following statements. I. T4 DNA ligase can catalyze blunt end Ligation more efficiently than E. coli DNA ligase. II. The ligation efficiency of T4 DNA Ligase can be increased with PEG and ficoll.