Vectors Uses for Animal Cell Culture MCQs

Question 1

Yeast episomal plasmids (YEp) were constructed using

Accepted Answer

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

Answer

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

Answer

12μm yeast plasmid and E coli vector pMB 9

Answer

12μm yeast plasmid and E coli vector pBR 322

Question 2

Which of the following is/are commonly used promoter of S. cerevisiae?

Accepted Answer

All of the above

Answer

Galactokinase (GAL 1)

Answer

Alcohol dehydrogenase II (ADH 2)

Answer

Phosphoglycerate kinase (PGK)

Question 3

Maximum amount of foreign DNA introduced into SV 40 viruses is

Accepted Answer

2.5 kb

Answer

10 kb

Answer

5 kb

Answer

1 kb

Question 4

Recombinant SV40 viruses introduce the foreign DNA into cells

Accepted Answer

without the DNA mediated transfection

Answer

with the DNA mediated transfection

Answer

with the RNA mediated transfection

Answer

without the RNA mediated transfection

Question 5

Which one of the following is the most stable vector?

Accepted Answer

YAC

Answer

YEp

Answer

YRp

Answer

YIp

Question 6

SV 40 vectors, in which early region replacements are carried out, require

Accepted Answer

COS cell line

Answer

SV 40 helper virus

Answer

pBR 322 vector

Answer

none of these

Question 7

Cloning genes in the yeast offer which of following advantage(s)?

Accepted Answer

All of the above

Answer

Over production of proteins of commercial value

Answer

Ability to glycosylate proteins during secretion

Answer

Ability to clone large piece of DNA

Question 8

Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Accepted Answer

Has a origin of replication and rep genes

Answer

Allows transformation by using crossing over

Answer

Carries autonomously replicating sequence

Answer

Carries centromere and telomere sequences

Question 9

Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Accepted Answer

All of the above

Answer

Contains centromeric sequences

Answer

Segregates during meiosis and mitosis

Answer

Functions as true chromosome