Vectors Uses for Animal Cell Culture MCQs

Question 1

Yeast Replicating plasmids (YRp) carry

Accepted Answer

autonomously replicating sequence

Answer

centromere

Answer

telomere

Answer

both (a) and (c)

Question 2

Which of the following viruses are used as vectors for transferring genes to animal cells?

Accepted Answer

All of these

Answer

SV 40 virus

Answer

Bovine papilloma virus (BVP)

Answer

Retroviruses

Question 3

Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

Accepted Answer

transcriptional regulators and are essential for viral replication

Answer

not necessary for viral replication

Answer

coding for virus coat protein

Answer

important for packaging of viral DNA into viral head

Question 4

Retroviruses have advantage for being used as vector for animal cells because

Accepted Answer

all of the above

Answer

they cover a wide host range

Answer

infection does not lead to cell death, infected cells produce virus over an indefinite period

Answer

viral gene expression is driven by strong promoters

Question 5

Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

Accepted Answer

either episomally or integrated at high copy number

Answer

episomally only

Answer

integrated only

Answer

either episomally or integrated at low copy number

Question 6

Which of the following histone is not present in SV40 virus?

Accepted Answer

H1

Answer

H2A

Answer

H3

Answer

H4

Question 7

Autonomously replicating sequence is not present in

Accepted Answer

YIp

Answer

YRp

Answer

YCp

Answer

YAC

Question 8

In case of Baculoviruses, the foreign gene

Accepted Answer

is inserted between the polyhedrin promoter and downstream sequences

Answer

is inserted downstream of polyhedrin promoter

Answer

is inserted downstream of viral origin of replication

Answer

is inserted randomly in viral genome

Question 9

α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Accepted Answer

Lys 2

Answer

His 3

Answer

Ura 3

Answer

Trp 1