Recombinant DNA MCQs

Question 1

Bacterial cells protect their own DNA from degradation by restriction endonucleases by

Accepted Answer

methylating the DNA at the sites that the enzyme recognizes

Answer

deleting all recognition sites from the genome

Answer

not producing any restriction endonucleases

Answer

having anti restriction endonucleases

Question 2

Which type of restriction endonuclease cuts the DNA within the recognition site?

Accepted Answer

Type II

Answer

Type I

Answer

Type III

Answer

All of the these

Question 3

The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

Accepted Answer

gene gun

Answer

laser

Answer

DNA probe

Answer

inoculating needle

Question 4

An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

Accepted Answer

a transgenic animal

Answer

a chimera

Answer

a vector

Answer

an enzyme that links DNA molecules

Question 5

The advantage of using DNA polymerases from thermophilic organisms in PCR is that

Accepted Answer

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

Answer

the DNA polymerases of these bacteria are much faster than those from other organisms

Answer

the DNA polymerases of these bacteria never make mistakes while replicating DNA

Answer

all of the above