Recombinant DNA MCQs

Question 1

What is the normal role of restriction endonucleases in bacterial cells?

Accepted Answer

To degrade invading phage DNA

Answer

To degrade the bacterial chromosome into small pieces during replication

Answer

To produce RNA primers for replication

Answer

All of the above

Question 2

Which of the following is not commonly used as vector?

Accepted Answer

Fungi

Answer

Artificial chromosome

Answer

Cosmid

Answer

Plasmid

Question 3

A short molecule containing 2-20 nucleotide is

Accepted Answer

oligonucleotide

Answer

plasmid

Answer

vector

Answer

mononucleotide

Question 4

Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

Accepted Answer

restriction endonucleases

Answer

DNA ligase

Answer

helicases

Answer

DNA gyrase

Question 5

Which of the following vector can maintain the largest fragment of foreign DNA?

Accepted Answer

YAC

Answer

Cosmid

Answer

Plasmid

Answer

Phage

Question 6

Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

Accepted Answer

gel electrophoresis

Answer

photoreactivation

Answer

autoradiography

Answer

blotting

Question 7

For gene probes to be useful they must

Accepted Answer

both (a) and (b)

Answer

be large enough to contain gene-specific sequences

Answer

be labeled in some manner to allow detection

Answer

none of the above

Question 8

A genomic library is

Accepted Answer

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

Answer

a database where the sequence of an organism's genome is stored

Answer

a book that describes how to isolate DNA from a particular organism

Answer

a place where the information of the genetic organization of organisms are kept.

Question 9

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

Accepted Answer

DNA ligase

Answer

DNA polymerase

Answer

Restriction endonuclease

Answer

DNA helicase