Recombinant DNA MCQs

Question 1

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

Accepted Answer

polymerase chain reaction

Answer

southern blotting

Answer

northern blotting

Answer

DNA replication

Question 2

The Southern blotting technique depends on

Accepted Answer

similarities between the sequences of probe DNA and experimental DNA

Answer

similarities between the sequences of probe RNA and experimental RNA

Answer

similarities between the sequences of probe protein and experimental protein

Answer

the molecular mass of proteins

Question 3

Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

Accepted Answer

expression vectors

Answer

BACS

Answer

YACS

Answer

all of these

Question 4

Agrobacterium tumefaciens is

Accepted Answer

a bacterium that can be used to introduce DNA into plants

Answer

a disease in humans that causes loss of sight

Answer

a fungi that is used to produce antibiotics in large amounts

Answer

a disease in humans that causes loss of weight

Question 5

In genetic engineering, a chimera is

Accepted Answer

a plasmid that contains foreign DNA

Answer

an enzyme that links DNA molecules

Answer

a virus that infects bacteria

Answer

a fungi

Question 6

The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

Accepted Answer

genetic engineering

Answer

population genetics

Answer

microbiology

Answer

protein engineering

Question 7

Electroporation is

Accepted Answer

the application of high voltage pulses

Answer

the process of separating charged molecules through a gel maintained in an electric field

Answer

the process of combining foreign DNA to an electrically charged vector molecule

Answer

the process of multiplication of the cells

Question 8

Which of the following is obtained using processed mRNA molecules as a template?

Accepted Answer

cDNA

Answer

rDNA

Answer

mDNA

Answer

tDNA

Question 9

Vectors are

Accepted Answer

molecules that are able to covalently bond to and carry foreign DNA into cells

Answer

molecules that degrade nucleic acids

Answer

molecules that help in replication

Answer

molecules that protect host cells from invasion by foreign DNA