Bioprocess Engineering and Process Biotechnology MCQs

Diauxic pattern of biomass growth is associated with P. Multiple lag phases. Q. Sequential utilization of multiple substrates. R. Simultaneous utilization of multiple substrates. S. Absence of lag phase.

Downstream processing of an industrial process yielded a highly purified bioactive protein. This protein was subjected to cleavage by trypsin. Chromatographic separation of products resulted in 4 peptides (P, Q, R, S) with the following amino acid sequences. P. Phe-Val-Met-Val-Arg Q. Ala-Ala-Try-Gly-Lys R. Val-Phe-Met-Ala-Gly-Lys S. Phe-Gly-Try-Ser-Thr Chemical cleavage of the same protein with cyanogen bromide and chromatographic separation resulted in three peptides (i, ii, iii) with the following sequences (i) Ala-Gly-Lys-Phe-Gly-Try-Ser-Thr (ii) Ala-Ala-Try-Gly-Lys-Phe-Val-Met (iii) Val-Arg-Val-Phe-Met The order of the peptides that gives the primary structure of the original protein is

Two monomeric His-tagged proteins of identical molecular weight are present in a solution. pIs of these two proteins are 5.6 and 6.8. Which one of the following techniques can be used to separate them?

Aeration in a bioreactor is provided by

The specific growth rate (μ) of a microorganism in death phase is

Immobilization of enzymes using entrapment method requires P. Photosensitive polyethylene glycol dimethacrylate. Q. CNBr activation of sepharose. R. Polyfunctional reagent like hexamethylene diisocyanate. S. Radiation of polyvinyl alcohol.

Which of the following techniques is not ideal for immobilizing cell-free enzymes?

Determine the correctness or otherwise of the following Assertion (A) and Reason (R). Assertion: Bacterial growth is called synchronous when majority of the cells are in same stage of the bacterial cell cycle. Reason: Synchronous culture can be obtained by growing bacteria in an enriched medium

An immobilized enzyme being used in a continuous plug flow reactor exhibits an effectiveness factor (η) of 1.2. The value of η being greater than one could be apparently due to one of the following reasons. Identify the correct reason.