Bioprocess Engineering and Process Biotechnology MCQs

If the dissociation constant for solute-adsorbent binding is KD, the retention time of the solute in a chromatography column

In large scale fermentation process, air is sterilized by

In large-scale fermentation, the preferred method of sterilization is

Phytase, an enzyme produced by Aspergillus niger can be adsorbed on microcrystalline cellulose powder (MCCP). The adsorption follows a Langmuir isotherm and the maximum concentration of the protein that can be obtained on the adsorbent is 70 mg cm-1. At a concentration of 50 mg L-1 of protein in the solution, the concentration of protein on the adsorbent reaches 35 mg cm-1. It is desired to recover 90% of the protein from 1.5 liter of the cell free culture filtrate containing 220 mg L-1 protein by addition of MCCP to the solution. The concentration of the protein adsorbed on the solid at equilibrium will be:

Match the entries in Group I with the process parameters in Group II. Group I Group II P. Clark electrode 1. Liquid level Q. Redox probe 2. Dissolved oxygen concentration R. Load cell 3. Vessel pressure S. Diaphragm gauge 4. pH (anaerobic process)