Bioprocess Engineering and Process Biotechnology MCQs

Question 1

Match each parameter in Group I with the appropriate measuring device in Group II. Group I Group II P. Pressure 1. Photometer Q. Foam 2. Rotameter R. Turbidity 3. Diaphragm gauge S. Flow rate 4. Rubber sheathed electrode

Accepted Answer

P-3, Q-4, R-1, S-2

Answer

P-1, Q-3, R-2, S-4

Answer

P-4, Q-1, R-2, S-3

Answer

P-1, Q-2, R-3, S-4

Question 2

To optimize the bioreactor system, which one of the following conditions is least important for anaerobic fermentation?

Accepted Answer

Culture agitation to maintain oxygen supply

Answer

Restriction of the entry of contaminating organisms

Answer

Control of parameters like pH and temperature

Answer

Maintenance of constant culture volume

Question 3

Determine the correctness or otherwise of the following Assertion (A) and Reason (R). Assertion: Immobilization of plant cells can enhance secondary metabolite production during bioreactor cultivation. Reason: Immobilization protects the plant cells from shear forces in the bioreactor.

Accepted Answer

Both (A) and (R) are true and (R) is the correct reason for (A)

Answer

Both (A) and (R) are true but (R) is not the correct reason for (A)

Answer

(A) is true but (R) is false

Answer

(A) is false but (R) is true

Question 4

Which of the following statements is incorrect about immobilized plant cell cultures?

Accepted Answer

It provides low shear resistance to cells

Answer

It is possible to use high cell densities

Answer

Cells remain active for long periods

Answer

Cell products or inhibitors can be removed easily

Question 5

Which one of the following relations holds true for the specific growth rate (μ) of a microorganism in the death phase?

Accepted Answer

μ < 0

Answer

μ = 0

Answer

μ = μmax

Answer

μ < μ < μmax

Question 6

Match the coefficients in Group I with their corresponding downstream processing Steps given in Group II. Group I Group II P. Sedimentation coefficient 1. Aqueous two-phase extraction Q. Partition coefficient 2. Ultrafiltration R. Rejection coefficient 3. Dialysis S. Activity coefficient 4. Centrifugation

Accepted Answer

P-4, Q-1, R-2, S-3

Answer

P-3, Q-1, R-4, S-2

Answer

P-2, Q-1, R-4, S-3

Answer

P-4, Q-3, R-1, S-2

Question 7

In chemostat operating under steady state, a bacterial culture can be grown at dilution rate higher than maximum growth rate by

Accepted Answer

Substrate feed rate cycling

Answer

Partial cell recycling

Answer

Using sub-optimal temperature

Answer

pH cycling

Question 8

Substrate consumption in lag phase of microbial growth is primarily used for P. Turnover of the cell material. Q. Maintenance of intracellular pH. R. Motility. S. Increase in cell number.

Accepted Answer

P, Q and R only

Answer

P, Q and S only

Answer

Q, R and S only

Answer

S only

Question 9

Cibacron Blue dye affinity chromatography can be used for affinity purification of

Accepted Answer

NADPH dehydrogenase

Answer

Glucoamylase

Answer

Subtilisin

Answer

Caspase