Bioprocess Engineering and Process Biotechnology MCQs

Question 1

Match the downstream processes in Group I with the products in Group II. Group I Group II P. Solvent extraction 1. Lactic acid Q. Protein-A linked affinity chromatography 2. Penicillin R. Extractive distillation 3. Monoclonal antibody d. S. Salting out 4. Lipase

Accepted Answer

P-2, Q-3, R-1, S-4

Answer

P-4, Q-1, R-2, S-3

Answer

P-4, Q-1, R-3, S-2

Answer

P-2, Q-4, R-1, S-3

Question 2

Which one of the following reactions is used for the purpose of recycling enzymes in bioprocesses?

Accepted Answer

Immobilization

Answer

Isomerization

Answer

Phosphorylation

Answer

Polymerization

Question 3

The factor(s) likely to increase the rate of reaction catalyzed by a surface immobilized enzyme is/are

Accepted Answer

Continued replacement of the bulk liquid containing the substrate

Answer

Increased agitation of the bulk liquid containing the substrate

Answer

Increased concentration of the substrate in the bulk liquid

Answer

All of the above

Question 4

The unit for specific substrate consumption rate in a growing culture is

Accepted Answer

$$\frac{g}{{g - h}}$$

Answer

$$\frac{g}{{L - h}}$$

Answer

$$\frac{g}{h}$$

Answer

$$\frac{{g\,{\mkern 1mu} moles}}{{L - h}}$$

Question 5

Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, ρ, of a double stranded Cs+ DNA is given by the equation ρ = 1.66 + 0.098 XG + C, where XG + C denotes

Accepted Answer

Mole fraction of G + C

Answer

Total number of G and C

Answer

Number of GC repeats

Answer

Ratio of G + C to A + T content

Question 6

Match the instruments in Group I with their corresponding measurements in Group II. Group I Group II P. Manometer 1. Agitator speed Q. Rotameter 2. Pressure difference R. Tachometer 3. Cell number S. Hemocytometer 4. Air flow rate

Accepted Answer

P-2, Q-4, R-1, S-3

Answer

P-4, Q-1, R-2, S-3

Answer

P-3, Q-4, R-1, S-2

Answer

P-2, Q-1, R-4, S-3

Question 7

Autoclaves are routinely used in laboratories for sterilization. They act by

Accepted Answer

All of the above

Answer

Disrupting cell membranes

Answer

Denaturing proteins

Answer

Changing physically membrane lipids

Question 8

Determine the correctness or otherwise of the following Assertion (A) and the Reason (R). Assertion: In synchronous culture, majority of the cells move to next phase of the cell cycle simultaneously. Reason: Synchronous culture could be obtained by starving cells for essential nutrient components.

Accepted Answer

Both (A) and (R) are true and (R) is the correct reason for (A)

Answer

Both (A) and (R) are true but (R) is not the correct reason for (A)

Answer

(A) is true but (R) is false

Answer

(A) is false but (R) is true

Question 9

In balanced growth phase of a cell, P. All components of a cell grow at the same rate. Q. Specific growth determined by cell number or cell mass would be the same. R. The growth rate is independent of substrate concentration. S. The growth rate decreases with decreasing substrate concentration.

Accepted Answer

Q, R and S only

Answer

P, Q and S only

Answer

P, Q and R only

Answer

P only