Bioprocess Engineering and Process Biotechnology MCQs

Evaluate the Michaelis constant for the following lipase catalyzed transesterification reaction for the production of biodiesel. $${\text{Vegetale oil}} + {\text{Lipase}} + \mathop \rightleftharpoons \limits_{{{\text{k}}1}}^{{{\text{k}}{ - 1}}} {\text{ Oil-lipase complex }}\mathop \to \limits^{{{\text{k}}_1}} {\text{Biodiesel}} + {\text{Glycerol}}$$ where k1 = 3 × 108 M−1 s−1; k−1 = 4 × 104 s−1 and k2 = 2 × 103 s−1

Which of the following is employed for the repeated use of enzymes in bioprocesses?

Choose the correct completion of the following statement. A fermenter sterilization in situ is less efficient than continuous heat sterilization because

Mechanism of separation of contaminants present in air by fibrous media are

Match the entries in the Group I with the elution conditions in Group II. Group I Group II P. Ion exchange chromatography 1. Isocratic solvent Q. Hydrophobic column chromatography 2. Ampholytes R. Gel filtration chromatography 3. Increasing gradient of salt S. Chromatofocusing 4. Decreasing gradient of polarity

Which of the following cases are likely to lead to faster rates of catalysis by an enzyme immobilized on a negatively charged support?

The Pasteur Effect is

Fed batch cultivation is suitable for which of the following? P. Processes with substrate inhibition Q. Processes with product inhibition R. High cell density cultivation

Ultrafiltration process cannot be used for