Animal Biotechnology MCQs

Somatic cell gene transfer is used for P. Transgenic animal production. Q. Transgenic diploid cell production. R. In vitro fertilization. S. Classical breeding of farm animals.

Cells deficient in hypoxanthine guanine phosphoribosyl-transferase (HPRT) enzyme rely on

An animal cell line was transected with DNA extracted from a tumorous tissue. Which one of the following will be diagnostic for its tumorous transformation?

Which are the most commonly used enzymes for separating cells in a given tissue for culture?

Bacillus subtilis under ideal conditions occupies 5% of the total volume of a fermenter. Under such conditions; the culture medium (after bacilli are filtered off) has a concentration of 1mg/ml of the secreted protein trehalae (i) Calculate the volume of culture filtrate required to purify 1 kg of trehalase (ii) What are the total number of B. subtilis cells which produce this amount of enzyme? Assume that each cell is a cylinder which has dimensions of 1 m diameter and length 2μm.

In animal cell cultures, the addition of serum to media is essential for providing

A knockout mouse is so named because it contains

In animal cell culture, a CO2 enriched atmosphere in the incubator chamber is used to maintain the culture pH between 6.9 and 7.4. Which one of the following statements is correct?

Which of these would not Be-used to introduce DNA in animal cells?