Animal Biotechnology MCQs

Question 1

What is the primary purpose of neomycin in creating mice with knockouts in gene X?

Accepted Answer

Neomycin selects for the survival of embryonic stem cells (ES) that have incorporated the mutant gene X anywhere in the genome

Answer

Neomycin selects for the survival of ES cell that have incorporated the mutant gene in the place of the wild-type gene

Answer

Neomycin prevents Candida infection during ES cell culture that does not have gene X

Answer

Neomycin makes the gene X knock-out mice resistant to Candida infection

Question 2

The growth medium for mammalian cells contains serum. One of the major functions of serum is to stimulate cell growth and attachment. However, it must be filter sterilized to

Accepted Answer

Remove mycoplasma and microorganisms

Answer

Remove large proteins

Answer

Remove collagen only

Answer

Remove foaming agents

Question 3

During the media preparation for cultivation of cells, insoluble precipitates of calcium phosphates are often formed, identify which method can be adopted to avoid this problem?

Accepted Answer

None of the above

Answer

Hold the pH at 5.6

Answer

Hold the pH at 7.5

Answer

Add calcium salt first and then phosphate source

Question 4

To create a transgenic organism:

Accepted Answer

Introduce foreign DNA into a gamete or fertilized ovum

Answer

Inject a gene of interest into a somatic cell

Answer

Inject a gene of interest into several somatic cells

Answer

Introduce foreign DNA into somatic cells in culture and transplant them

Question 5

When scientists make an animal superior by view of genotype, introducing some foreign genes in it, the phenomenon is called

Accepted Answer

Genetic engineering

Answer

Tissue culture

Answer

Biotechnology

Answer

Immunisation

Question 6

The following culture systems are used for growing large amount of anchorage dependent animal cells except

Accepted Answer

Airlift fermenter

Answer

Roller bottle

Answer

Hollow fiber reactor

Answer

Microcarriers

Question 7

Which of the following promotes glucose and amino acid uptake by muscle?

Accepted Answer

Insulin

Answer

Adrenaline

Answer

Glucagon

Answer

Cortisol

Question 8

Mitochondrial DNA is replicated from

Accepted Answer

Two different ori sites at different times in opposite directions

Answer

A single ori site bidirectionally

Answer

Two different ori sites in the same direction

Answer

Many sites bidirectionally, like nuclear chromosomes

Question 9

In transgenics, alteration in the sequence of nucleotide in genes are due to I. Subsitution II. Deletion II. Insertion IV. Rearrangement

Accepted Answer

I, II and III

Answer

I and II

Answer

II and III

Answer

III and IV