Media Required for Plant Cell Culture MCQs

Question 1

Concentration of sucrose generally used in plant cell culture media is

Accepted Answer

20-30 g/l

Answer

10-15 g/l

Answer

40-50 g/l

Answer

60-70 g/l

Question 2

Which is/are the naturally occurring plant auxins?

Accepted Answer

Indole acetic acid (IAA)

Answer

Naphthalenacetic acid (NAA)

Answer

2,4-dichlorophenoxyacetic acid

Answer

All of the above

Question 3

Which is/are the disadvantage/(s) of using IAA in plant cell culture media?

Accepted Answer

All of the above

Answer

It is unstable in solution

Answer

Gets easily oxidized

Answer

Conjugated to inactive form by plant cells

Question 4

To maintain the pH of the culture

Accepted Answer

both (a) and (b)

Answer

organic acid such as citric, fumaric, malic and succinic acid is used

Answer

synthetic buffers such as Tris, MES or HEPS are used

Answer

ammonium salts are used

Question 5

Which of the following is not a cytokinin?

Accepted Answer

2,4-dichlorophenoxyacetic acid

Answer

6 benzylaminopurine

Answer

Zeatin

Answer

Kinetin

Question 6

Which of the following is not an auxin?

Accepted Answer

zeatin

Answer

Indole acetic acid (IAA)

Answer

Naphthalenacetic acid (NAA)

Answer

Indole butyric acid

Question 7

Which of the following is not true about nurse or conditioned medium?

Accepted Answer

it is removed aseptically from the culture and is autoclaved before use

Answer

It is liquid removed from the suspension of fast growing cells

Answer

It contains uncharacterized growth factor released by growing cells

Answer

It is used in the culture of regenerating protoplast

Question 8

Very high sugar concentration (40-100 g/l) have been used

Accepted Answer

Both (a) and (b)

Answer

in specialized secondary metabolite production

Answer

to adjust the osmotic potential of the media in short term treatment for regeneration

Answer

none of these

Question 9

What is 'nurse' or conditioned medium?

Accepted Answer

It is the liquid medium removed from the suspension of fast growing cells

Answer

It is the media full of growth factors used for the growth of cells

Answer

It is the medium added to nurse the callus culture

Answer

Both (a) and (b)