Basic Principles of Biotechnology MCQs

Question 1

Microsatellites are used more commonly than minisatellite as DNA markers because

Accepted Answer

Microsatellites are present throughout eukaryotic genomes and are easily amplified using PCR

Answer

Minisatellites are present in too many locations within the genomes

Answer

Restriction enzymes can be used to type microsatellites but not minisatellite

Answer

There are very few microsatellites in eukaryotic genomes so they are easily identified and analyzed

Question 2

Which of the following Chemotherapeutic agents works by impairing de novo synthesis?

Accepted Answer

Methotrexate (antifolate)

Answer

Acyclovir (acycloguanosine)

Answer

5-fluorouracil (antimetabolite)

Answer

Hydroxyurea

Question 3

A multiple cloning site

Accepted Answer

Allows flexibility in the choice of restriction enzymes for cloning

Answer

Contains many copies of a cloned gene

Answer

Allows flexibility in the choice of organism for cloning

Answer

Contains many copies of the same restriction enzyme site

Question 4

X-ray crystallography are P. Based on the elastic scattering of X-rays Q. Non-destructive analytical techniques R. Used in determining the arrangement of atoms within a crystal S. Based on Bragg's law

Accepted Answer

All of the above

Answer

P and Q

Answer

R and S

Answer

P, Q and S

Question 5

Phase contrast microscope takes advantage of

Accepted Answer

Density of different parts of a cell

Answer

Volume of a cell

Answer

Size of nucleus

Answer

None of the above

Question 6

Measurement of KLa in a bioreactor can be carried out by sodium sulfite oxidation method, that is, based on the oxidation of sodium sulfite to sodium sulfate in the presence of a catalyst (Cu++ or Co++). In a typical experiment, a laboratory fermenter was filled with 5 liter of 0.5 M sodium sulfite solution containing 0.003 M Cu++ ions and the air was sparged in. After 10 min, the air flow was stopped and a 10 mL sample was taken and titrated. The concentration of sodium sulfite in the sample was found to be 0.20 M. The oxygen uptake rate for this aerated system will work out to be

Accepted Answer

0.008 g L-1 s-1

Answer

0.08 g L-1 s-1

Answer

0.8 g L-1 s-1

Answer

None of the above

Question 7

In a well aerated and agitated microbial culture, the 'supply' of oxygen is equal to 'demand' (uptake) of the growing culture. The KLa for such a system will be (KLa = volumetric mass transfer coefficient, C* = dissolved oxygen concentration in liquid in equilibrium with gaseous oxygen, C = instantaneous value of dissolved oxygen concentration, 'r' = specific oxygen uptake rate per unit weight of cells, X = dry weight of the cells per unit volume)

Accepted Answer

$$\frac{{\left( {rX} \right)}}{{\left( {C* - C} \right)}}$$

Answer

$$\frac{{\left( r \right)}}{{X\left( {C* - C} \right)}}$$

Answer

$$\frac{{\left( {C* - C} \right)}}{{\left( {rX} \right)}}$$

Answer

$$\frac{{\left( X \right)}}{{r\left( {C* - C} \right)}}$$

Question 8

Glycogen is a branched polymer of glucose It has

Accepted Answer

One reducing end and several non-reducing ends

Answer

No reducing ends

Answer

No non-reducing ends

Answer

One non-reducing end and several reducing ends

Question 9

Shear stress versus shear rate behavior of four different types of fluids (I, II, III and IV) are shown in the figure below. Which one of the following options is correct?

Accepted Answer

I-Newtonian, II-Bingham plastic, III-Pseudoplastic, IV-Dilatant

Answer

I-Newtonian, II-Bingham plastic, III-Dilatant, IV-Pseudoplastic

Answer

I-Pseudoplastic, II-Dilatant, III-Newtonian, IV-Bingham plastic

Answer

I-Newtonian, II-Pseudoplastic, III-Bingham plastic, IV-Dilatant