Basic Principles of Biotechnology MCQs

Question 1

Which of the following statements are correct? Very long DNA fragments (> 100 kb) from eukaryotic genomes P. Can be efficiency packaged in a λ phage vector Q. Can be propagated in yeast artificial chromosomes R. Must have cohesive ends for cloning S. Can be separated by pulsed field gel electrophoresis techniques

Accepted Answer

Q and S

Answer

P and Q

Answer

P ans S

Answer

Q and R

Question 2

Which one of the following statements about eukaryotic RNA polymerases I, II and III is false?

Accepted Answer

The carboxyl end of RNA Pol II contains a short sequence of only seven amino acids which is called the CTD and which may be phosphorylated

Answer

RNA Pol II is very sensitive to α-amanitin

Answer

RNA Pol II is located in the nucleoplasm

Answer

RNA Pol III transcribes the genes for tRNA

Question 3

Henderson's equation can be respresented as

Accepted Answer

$$pH = p{K_a} + log\frac{{[Salt]}}{{[Acid]}}$$

Answer

$$pH = p{K_a} + log\frac{{[Acid]}}{{[Salt]}}$$

Answer

$$p{K_a} = pH + log\frac{{[Salt]}}{{[Acid]}}$$

Answer

$$p{K_a} = pH - log\frac{{[Salt]}}{{[Acid]}}$$

Question 4

The role of calcium in muscle contraction is to

Accepted Answer

Bind with troponin, changing its shape so that the actin filament is exposed

Answer

Break the cross-bridges as a cofactor in the hydrolysis of ATP

Answer

Transmit the action potential across the neuromuscular junction

Answer

Spread the action potential through the T tubules

Question 5

Why is the inactivation of a useful technique to determine the function of a gene?

Accepted Answer

Gene inactivation provides an opportunity to identify phenotypic changes associated with the loss of the functional gene

Answer

Gene inactivation provides information about the expression of a gene

Answer

Gene inactivation provides information about the cellular location of a gene product

Answer

Gene inactivation provides information about the structure of the gene product

Question 6

Which of the following statements is not true of fatty acids found in most mammalian cells?

Accepted Answer

Polyunsaturated fatty acids contain conjugated double bonds

Answer

They usually contain 12 to 20 carbons

Answer

All double bonds are in the cis configuration

Answer

Free fatty acids are ionized at physiological pH

Question 7

The isoelectric point of a protein is defined as

Accepted Answer

The pH at which the net charge on the molecule is zero

Answer

The pH at which all groups are protonated

Answer

The pH at which all groups are unprotonated

Answer

The pH at which each acidic group is protonated and each basic group is unprotonated

Question 8

Prandtl number is the ratio of

Accepted Answer

Momentum diffusivity to thermal diffusivity

Answer

Thermal diffusivity to momentum diffusivity

Answer

Mass diffusivity to momentum diffusivity

Answer

Thermal diffusivity to mass diffusivity

Question 9

Energy capture efficiency of the aerobic cells using glucose as a substrate is

Accepted Answer

40%

Answer

50%

Answer

30%

Answer

20%