Analytical Tools in Biotechnology MCQs

Question 1

To tolerate high operating temperatures, which of the following has to be done in incandescent or tungsten filament lamps?

Accepted Answer

Envelope is fabricated with quartz

Answer

Alloys must be used

Answer

Nitrogen be used instead of inert gas

Answer

Envelope is fabricated with copper

Question 2

The helix content of a protein can be determined using

Accepted Answer

A circular dichroism spectrometer

Answer

An infrared spectrometer

Answer

A fluorescence spectrometer

Answer

A UV-Visible spectrophotometer

Question 3

How can the intensity of radiation be increased in Hollow cathode lamp?

Accepted Answer

Addition of non-conductive protective shield of mica

Answer

Addition of nitrogen to neon or argon in the lamp

Answer

Increasing the pressure of the filling gas

Answer

Changing the metal of the anode

Question 4

Tertiary structure of a protein consisting of α-helices and β-strands can be determined by

Accepted Answer

Nuclear magnetic resonance spectroscopy

Answer

Circular dichroism spectroscopy

Answer

Mass spectrometry

Answer

UV spectroscopy

Question 5

Which of the following is the advantage of grating monochromators?

Accepted Answer

Dispersion occurs in a linear manner

Answer

Dispersion is non-overlapping

Answer

Dispersion occurs in non-linear manner

Answer

Dispersion is overlapping

Question 6

In Atomic Absorption Spectroscopy, which of the following is the generally used radiation source?

Accepted Answer

Hollow cathode lamp

Answer

Tungsten lamp

Answer

Xenon mercury arc lamp

Answer

Hydrogen or deuterium discharge lamp

Question 7

Which of the following is not a technique for preparing solid samples in IR spectroscopy?

Accepted Answer

Thin films

Answer

Solids run in solution

Answer

Mull technique

Answer

Solid films

Question 8

Which of the following options are correct in terms of wavelength for the different types of IR spectrometer?

Accepted Answer

Near IR: 0.8 - 2.5 mm

Answer

Mid IR: 0.8 - 2.5 mm

Answer

Far IR: 2.5 - 50 mm

Answer

Mid IR: 50 - 100 mm

Question 9

Which of the following is the principle of Atomic Absorption Spectroscopy?

Accepted Answer

Radiation is absorbed by non-excited atoms in vapour state and are excited to higher states

Answer

Medium absorbs radiation and transmitted radiation is measured

Answer

Colour is measured

Answer

Colour is simply observed