Recombinant DNA Technology MCQs

Expression in poor amount and in inactive form of cDNA of a eukaryotic protein in Escherichia coli using its expression vector is due to P. The absence of capping mechanism of mRNA. Q. Codon bias. R. Absence of polyadenylation. S. Absence of proper glycosylation.

T4 polynucleotide kinase is used for

Restriction endonucleases which recognize and cut same recognition sequences are known as

The length of each boarder sequence in Ti plasmid is about

A DNA fragment of 4500 bp has to be tailed with dT residues by using dTTP and the enzyme 'terminal transferase' The stock solution of dTTP that is used as a substrate has concentration of 150 μM. Ten μL of this stock solution is added to a total volume of 200 μL reaction. What will be the concentration of dTTP in the reaction?

Baculovirus expression system is used to express heterologous genes in

A variety of genetic elements are used in the transgenic plant research. Match the genetic elements (Group I) with their corresponding source (Group II). Group I Group II P. Ubiquitin1 promoter 1. Agrobacterium tumefaciens Q. Nos transcriptional terminator 2. Streptomyces hygroscopicus R. bar selection marker gene 3. Escherichia coli S. gus reporter gene 4. Zea mays

The essential component of Ti plasmid required for integration into plant genome is

The bacteria known to be naturally competent for transformation of DNA is